88: SULFOLOBUS MEDIUM

Modifications: Supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and ore by heating at 150°C overnight. Add sulfur and ore aseptically to the autoclaved medium. Adjust the pH of the medium to 3.0.

Growth observed: (+)

Growth conditions: 2-3 days 70 °C